Dr Daniel J. Guerra

Authentic Biochemistry

Science EN ↓ 1250 episodes

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Dr Daniel J. Guerra

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Anhedonic and MDD- Associated Appetitive Reward Pathway Biochemistry and Pathophysiology II. DJGPhD. Authentic Biochemistry.30..12.21. 31.12.2021 29:50

References Front Neurosci. 2019; 13: 707 Nature Metabolism 2019.  1:754–764  Int. J. Mol. Sci. 2019, 20(6), 1505 Neuropsychiatr Dis Treat. 2015; 11: 875–888 Mol Psychiatry. 2016 Oct; 21(10): 1358–1365.

Appetitive Reward Pathway Biochemistry and Neural Circuitry Regulation I. Dr. Daniel J. Guerra. 29.12.21. An Authentic Biochemistry Production. 29.12.2021 30:00

References Biol Psychiatry. 2020 Jan 1; 87(1): 64–73 Front. Neurosci., 26 November 2019

AMPK and Immuno-Epigenetic Biochemistry Part 2/2/ DJGPhD. Authentic Biochemistry 27.12.21 28.12.2021 29:49

J Neuroinflammation. 2019; 16: 34 Nature Cell Biology, 02 Sep 2011, 13(9):1016-1023 Critical Reviews in Clinical Laboratory Sciences Volume 56, 2019 - Issue 7 Pages 472-492

AMPK and Immuno-Epigenetic Biochemistry Part 1. Dr. Daniel J. Guerra. 27.12.21. Authentic Biochemistry 27.12.2021 29:59

References Nat Cell Biol. 2019 Mar; 21(3): 397–407 CELL Volume 126, Issue 3, 11 August 2006, Pages 503-514 Prostaglandins Leukot Essent Fatty Acids. 2015 Jul;98:49-55 Int J Biol Sci. 2015; 11(11): 1272–1280 Sci Signal. 2017 Jan 31; 10(464): eaaf7478. Front Immunol. 2019; 10: 345. J Neuroinflammation. 2019; 16: 34.

AMPK negatively regulates lipogenesis by phosphorylating ACCase 1&2 plus HMGCoA reductase thus supporting beta oxidation of fatty acids . Dr. Daniel J. Guerra. 22.12.21. Authentic Biochemistry 23.12.2021 29:54

Cell Metabolism 2020 31472-492 Amino Acids. 2012 Oct;43(4):1803-7 Molecular Cell Volume 81, Issue 18, 16 September 2021, Pages 3677-3690

AMPK is associated with the POMC vs, NPY neuronal axis under Leptin Control. DJGPhD. 20.12.21 Authentic Biochemistry 21.12.2021 29:59

References HORMONES 2013, 12(2):172-191 Proc Natl Acad Sci U S A . 2002 Dec 24;99(26):17155-60 Eur J Endocrinol. 2017 176(5): R235–R246.

AMPK Allosteric Regulation and Interaction with the Opposing mTORC Network. Part 2. Dr. Daniel J. Guerra. Authentic Biochemistry 19.12.21 20.12.2021 30:00

Eur J Endocrinol. 2017 176(5): R235–R246 Molecular Cell Volume 81, Issue 18, 16 September 2021, Pages 3677-3690

Bio-Molecular Regulator Series . AMPK. Part One. Dr. Daniel J. Guerra. Authentic Biochemistry Podcast Production.19.12.21. 19.12.2021 30:00

An introductory lecture on the biomolecular sensor kinase, Adenosyl Mono-Phosphate (protein) Kinase;  AMPK. References Mol Genet Metab. 2013 Jul;109(3):260-8 PLoS One. 2016 Aug 16;11(8):e0161252. Experimental & Molecular Medicine volume 48, 224 (2016) Molecular Cell Volume 81, Issue 18, 16 September 2021, Pages 3677-3690

T-lymphocyte and macrophage membrane CD38 ecto-enzyme mediated NAD catabolism is associated with ageing and the decline in Sirtuin-mediated deacylase activity. DJGPhD.11.Dec.21' Authentic Biochemistry 12.12.2021 29:57

References Cell Metabolism. 2016. 23, 1127–1139 Cells 2020, 9(1), 228 Front. Immunol., 31 May 2019 Int J Mol Sci. 2020 Nov; 21(22): 8609 Circ Res. 2018 Sep 14; 123(7): 868–885. Int J Mol Sci. 2019 Mar; 20(5): 1223

Aposteriori synthesis of neuroendocrine linked clock chronicity and the Senescent associated Secretory Phenotype through the synthetic apriori lens of NAD+. DJGPhD.10Dec21. Authentic Biochemistry 10.12.2021 29:47

References Nat Cell Biol. 2019 Mar; 21(3): 397–407 J Diabetes Metab Disord. 2020 Jul 21;19(2):1797-1825 Int. J. Mol. Sci. 2014, 15, 16848-16884

How testosterone-aromatase derived estradiol transformation to 2-methoxy-17beta-estradiol can mitigate cell proliferation by promoting a senescent phenotype.DJGPhD. Authentic Biochemistry.8.DEC.21 08.12.2021 30:03

Besides 2-methoxy-17beta-estradiol, cell cycle arrest via chromatin deacetylation  can mitigate cell proliferation by decreasing global transcription while promoting the expression of tumor suppressor genes like p16INK4a while DNA Damage Repair (DDR) from pro-inflammatory cytokine production from senescent cells and Th1 lymphocytes can  inhibit commitment to mitosis and cytokinesis by ge...

Blocking cellular senescence may suppress autoimmune diseases and reactive oxygen mediated cell death but non-ironically promote oncogenesis and tumor development during ageing. DJGPhD. 06.DEC.21 07.12.2021 29:56

Cell fate from Either ageing associated senescence to programmed cell death OR cell proliferation is finely tuned to the splice variant  expression of Cell Cycle dependent kinase inhibitors and the relative production of pro-inflammatory cytokines under the synchrony of DNA Damage Repair (DDR), telomerase expression decline, and the protective role of the Senescence Associated Secretory Pheno...

Ageing and Asymmetric Dimethylarginine (ADMA)in Endothelial Nitric Oxide Synthase inhibition-associated decrease in respiratory vasodilation. Dr Dan Guerra 24.11.21 Authentic Biochemistry 25.11.2021 29:37

Age Ageing , Volume 48, Issue 6, November 2019, Pages 776–782 Biomedicines. 2020 Aug 6;8(8):277

Closing the window and drawing the curtain: cancer phenotypes are resolute signatures of Immunosenescence and inflammaging induced epigenetic collapse of vital biochemical homeostasis. DJGPhD.20/11/21 21.11.2021 29:29

Dr. Guerra authentically arrives at the inveighing cancer paradigm contribution to the sickness unto death.

Sealing Fate: Immunosenescent and inflammatory mechanisms combine the ineffectual with the increscent pathophysiology of sickness unto death. DJGPhD. 17.11.21 18.11.2021 29:16

Nutrients. 2020 Mar; 12(3): 622. Biochemistry and Cell Biology of Ageing: Part II Clinical Science. 2019. pp 21-43.The Immune System and Its Dysregulation with Aging Ludmila Müller, Svetlana Di Benedetto, and Graham Pawelec. Springer Publishers

The interplay between Immunosenescence and inflammaging conduct the innate and acquired immune response toward functional cellular rigidity and incompetence obtaining morbidity and mortality. DJGPhD. 14.11.2021 29:52

1.Biochemistry and Cell Biology of Ageing: Part II Clinical Science. 2019. pp 21-43. The Immune System and Its Dysregulation with Aging.  Ludmila Müller, Svetlana Di Benedetto, and Graham Pawelec. Springer Publishers. 2. Aging (Albany NY). 2019 Apr 30; 11(8): 2343–2351. 3. Immun Ageing. 2020; 17: 2

Fine detail of Treg cell control over inflammation suppression via FoxP3 transcriptional modulation. Aging and Obesity will limit this control leading to hyperinflammation. DJGPhD. 08Nov2021 09.11.2021 29:59

References Front. Immunol., 29 June 2012  J Immunol 2016;197:3762-3770 Front. Immunol., 03 February 2020

T cell receptor signaling kinase dynamics after APC activation. Co-activating CD28 becomes deficient via immunosenescence thus corrupting T cell efficiency in the elderly. 26.10.2021 DJGPhD. 27.10.2021 29:28

The Src-family kinase Lck regulates T-cell development, activation, proliferation, and immune synapse dynamics. These are the initiation phases of TCR  signaling. Upon antigen presentation via the APC- TCR immune synapse, Lck phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAMs) of the TCR-associated CD3 membrane protein domains.  Phosphoryla...

Indeed oxysterol LDL , circulating hepatic-derived convertase, T lymphocyte differentiation and macrophage to foam cell degeneration obtain the aging cardiovascular/pulmonary pathophysiological state. 22.10.2021 29:57

Dr Guerra carries out the synthesis of multiple pathobiochemical events into an architechtonic of the aging human co-morbidity resulting in a sickness unto death. Authentic Biochemistry Production 22 October 2021 References Cardiovasc Diabetol. 2020; 19: 33. Cytometry A. 2006 Mar;69(3):189-91 Nat Rev Dis Primers. 2019 Mar 7;5(1):16 J Lipid Res. 2007 Jan;48(1):19-29. doi: 10.1194 Mech Ageing Dev. 2...

How Oxysterol LDL associates with macrophage and T lymphocyte infiltration during aging to generate the hyperinflammatory atherosclerotic plaque. DJGPhD. 19.10.21. Authentic Biochemistry. 20.10.2021 29:33

That there are senescence-associated  decreases in the JAK-STAT signaling transduction cascade has been observed in human lymphocyte lineages. This phenomena, as  associated with a decreased JAK- tyrosine phosphorylation of STAT5, is linked to plasma membrane cholesterol content. Therefore, plasma membrane cholesterol content is involved in T cells modulation and proliferation. Acid sphi...

Aging results in corruption of T lymphocyte differentiation associated with chromatin instability and epithelial to mesenchymal transitions leading to the pre-cancerous state. DJGPhD 16.10.21 16.10.2021 29:52

Chromatin instability leads to alterations in gene expression that re-directs cellular senescence by corruption of chemokine and cytokine signalling resulting in epithelial to mesenchymal transitions and the potential for metastatic cancer in the elderly . As lymphocyte lineage's age these underlying pathobiochemical events are potentiated by hypo and hyper immune responses thus generating a downw...

Lymphocyte maturation and the special Innate like Lymphocyte Subclass including Natural Killer and other ILC lineages. DJGPhD.14.10.21. Authentic Biochemistry 14.10.2021 29:59

Front Cell Dev Biol . 2019; 7: 226 Advances in Immunology. 2011 v.109.Pp125 Cell Reports 10, 2043–2054, March 31, 2015 Experimental & Molecular Medicine volume 51, pages1–15 (2019)

Immunosenescence and Inflammaging via thymic Involution and comorbid pathophysiology of obesity and aging present a chronic inflammatory phenotype prone to morbidity. DJGPhD. Oct.04,2021. Authentic BC 05.10.2021 30:00

Dr. Guerra details the aging immune phenotype at the transcriptional and metabolic level. References Immun Ageing. 2020; 17: 2. Experimental & Molecular Medicine volume 51, Article number: 80 (2019) Nat Immunol. 2011 Nov 6; 12(12): 10 Cell 146, 772–784, September 2, 2011 Nat Immunol. 2014 Aug; 15(8): 767–776. Cell Reports Volume 17, Issue 11, 13 December 2016, Pages 2827-2836

Natural cytotoxicity receptors (NCR's) within group 3 innate lymphoid cells (ILC3): an association of transcription factors in IBD's and aging. DJGPhD. 03.10.21 Authentic Biochemistry. 04.10.2021 29:58

ILC3 can contribute to the progression and aggravation of IBD's while both IL-22 and IL-17, along with IFN-γ, are overexpressed by the dysregulation of NCR− ILC3 or NCR+ ILC3 function NCR (natural cytotoxicity receptors), are  type 1 transmembrane proteins of the immunoglobulin superfamily. Upon stimulation, NCR's mediate NK killing and release of IFNγ. They bind viral ligands such as hemaggl...

Omega three fatty acids and PE N-methyl transferase are associated with methylation and acetylation patterning in NK cells via AMPK and stress.DJGPhD. 29 September 2021. 29.09.2021 29:50

Omega-Three VLCPUFA's as asymmetrically distributed in membrane glycerolphospholipids mediate vesiculation and permeability of endothelia to circulating lymphocytes and leukocytes thus coupling folic acid, Vitamin B12 and epigenetic modification of chromatin to ultimately link bioenergetics with cytotoxicity and inflammation. This regulated system involves the neuroimmunoepigenome and the senescen...