Listen In - Bitesize Bio Webinar Audios

In this webinar, you will learn how synthetic biology can be used to engineer desirable and robust behaviors or traits into model organisms. The main aspects to be covered in this webinar include: Introduction to synthetic biology – what it is and what it’s not Popular applications for synthetic biology in research The pros and cons of synthetic biology Tips and resources for getting started in th...

Expression and Purification of an Engineered, E. coli-expressed Leishmania donovani Nucleoside Hydrolase with Immunogenic Properties Potential recombinant protein vaccine candidates must meet several criteria: They must be expressed at sufficiently high levels in the organism of choice They must be purified to high purity from the expression system in an immunogenic form They must induce potent im...

In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research. The main points in the webinar will include: How to design guide RNAs using online tools specific to the genome and application of interest. Tips and practical advice to assist you in choosing and optimizing a CRISPR/Cas9 delivery system. How you can cr...

Join Dr. Karen O’Hanlon Cohrt for a practical tour of multiplex PCR technology, where you will learn the following and much more: Principle behind multiplex PCR technology Popular applications of this technology How to set up this reaction Advantages and disadvantages Multiplex PCR Can Benefit Your Research Dr O’Hanlon Cohrt will discuss the history of multiplex PCR, how the technique works, and h...

The rise of open access is changing how research is communicated. In this webinar, we’ll celebrate Open Access Week 2016 by taking a closer look at how open access affects how researchers write and publish their results. Specifically, we will: Define open access and Creative Commons licensing and dive into the numbers about open access (journals, articles, fees) Discuss the pros and cons of open a...

In this webinar, you will learn about color reproduction in microscopy images—and how to fix it if your color reproduction goes awry. The main points we will cover are: The reasons that could color reproduction go wrong in your microscopy images How to correct your microscopy images with inappropriate color reproduction How to be ethical with the color in your microscopy images Advice on Color in...

Incorporating New Dyes to Simplify Panel Design: Accelerating Reagent Availability In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components. We will cover: How quality custom reagents in small sizes will make your panel design easier How you can receive these reagents fast and without traditional wait times for...

In this webinar, you will learn about the process for genotyping cells—particularly tumor cells—and practical advice for analyzing the resulting data. The main points that we will cover are: Practical advice and methods for sample collection and preparation for your genotyping experiments An overview of the available data analysis methods for your genotyping results Examples of pathway analysis me...

In this webinar you will learn the tips and tricks necessary for you to successfully work with miRNAs, and how you can use them to further your research. The main points we will cover are: Tips and practical advice to help you work with miRNAs, including isolation and delivery The biology and mechanism of miRNAs and why this matters to your research An example of how miRNAs are being used in disea...

Tools for Analyzing Genetic Variants froEvery individual harbors millions of genetic variants, many of which may contribute to phenotype. As the cost of sequencing has plummeted, we now have the opportunity to profile this variation across thousands of individuals. Here I will provide an overview of the workflow to analyze various types of genetic variation, starting from raw sequencing data and e...

The current state of the academic job market has come under scrutiny in recent years, largely because of the shrinking number of tenure-track jobs available. This situation has forced graduate students and postdocs to reconsider their employment options. Other scholars may realize that academia is no longer a good fit for them but are unsure of how to land a non-faculty position. This process of s...

Next generation sequencing (NGS) is a powerful tool for investigating genome-wide phenomena. It has been used to study epigenetic profiles as part of the ENCODE project. It is being used to understand the genetic basis of both common and rare diseases. The Cancer Genome Atlas (TCGA) used NGS to map hundreds of cancer genes. There is no area of modern genetic research that has not been transformed...

Whether it’s equipment costing hundreds of thousands of dollars and requiring millions of dollars in R&D investments or standardized kits that relieve you of tedious grunt work day in and day out, you rely on the support of companies like our sponsors to provide you with the equipment and supplies you need. But beyond the things they sell, these companies know a thing or two about your work-a-...

All mRNA molecules recruit specific proteins to form ribonucleoprotein complexes (mRNPs). Composition and localization of many mRNPs change dynamically from translation to decay. Microscopic techniques with high spatial and temporal resolution are invaluable for studying mRNP biogenesis. We have developed new tools based on fluorogenic forced intercalation (FIT) probes for RNA detection, quantific...

We know that cellular heterogeneity in the tumor microenvironment complicates the diagnosis and treatment of cancer. Tumors are complex, dynamic systems composed of diverse cell types in various functional states, including cancer cells and infiltrating immune cells. Resolving this cellular heterogeneity requires a single-cell approach, as analyses on bulk cell preparations mask the heterogeneity...

Biobanks, or biorepositories, store biological samples that are critical for research in the biomedical fields. Samples from biobanks have been used for genomics research, investigations into the development of personalized medicine, and treatment of diseases. After obtaining precious specimens, it is important to not only store them for easy retrieval, but is also vital to treat the samples so th...

Leica Microsystems Laser Microdissection systems are the method of choice for laser-based microscopic sample dissection and collection. Laser Microdissection (LMD) is a microscopic technique for isolating homogeneous, specific and pure targets from heterogeneous samples for downstream analysis (DNA, RNA & proteins). In addition, the Leica Microsystems LMD systems can be used with live cell cul...

Enjoying your work should be one of life’s top priorities not only because you spend much of your time working, but also because if you enjoy your work you’ll inevitably do a better job. Having worked in an uptight academic lab, a very laid-back Danish biotech company, a typically British biotech lab and been my own working-from-home boss for a number of years I have tried many different approache...

Seeing and believing that the dress is yellow may not be that important for the casual wedding image, but in microscopy imaging seeing and believing is critical. Microscopy images that contain color, such as images from histology­-stained specimens, must be compared and analyzed. If the color reproduction is poor, then there’s a chance that important information will be overlooked, not seen, and n...

Living cells and organisms often suffer from the high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signal is detected in a perpendicular direction. In combination with high-speed cameras for image acquisition, light sheet microscopy is a very gentle me...

In this webinar, you’ll get: Practical advice for sample preparation, qPCR setup and result analyses Guidance on choosing the correct fluorescent labeling system for your qPCR Tips to troubleshoot the most commonly encountered issues in qPCR Join Dr. Karen O’Hanlon Cohrt for a practical tour of the process, applications and practice of qPCR, from start to finish. Beginning with an overview of how...

Recently, there has been a push to develop alternative methods to traditional invasive techniques, such as solid tissue biopsies, for disease diagnosis and disease progression, as well as therapeutic response. Liquid biopsy is a new, minimally invasive technology for detecting circulating biomarkers without the need for costly or invasive procedures. Liquid biopsy enables users to sensitively, spe...

The Fast Module for ZEISS LSM 880 with Airyscan: Confocal Superresolution Imaging with Four Times the Speed and Improved Signal-to-Noise Ratio First introduced in August 2014, the Airyscan detector from ZEISS represents a new detector concept for laser scanning microscopy (LSM) that enables a simultaneous resolution and signal-to-noise (SNR) increase over traditional LSM imaging. The Airyscan dete...

Chromatin Immunoprecipitation (ChIP) is a powerful technique for evaluating the interactions of proteins with specific regions of genomic DNA, helping to better understand the mechanisms such as, gene regulation, DNA replication, repair and recombination, and epigenetic silencing. The use of ChIP in conjunction with NGS (ChIP-Seq) has enabled wide scale application of this technique to ascertain g...