Listen In - Bitesize Bio Webinar Audios

Join us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn: – How to choose the best method to extract your nucleic acids – Which method you should chose to determine nucleic acid quality – How to avoid common pitfalls for both extraction and quality control Virtually all experiments in the molecular biology lab require hi...

In this webinar, you will learn: – How to best prepare your specimen for live cell isolation by laser microdissection – How to optimize your laser microdissection workflow – How to avoid common pitfalls of this technique Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fix...

In this webinar, you will learn: – How to overcome issues with stage drift and crosstalk between channels – How to obtain reliable images and reproducible quantification results – How to proceed with advanced image analysis after restoring images During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blu...

Join us in this webinar featuring Dr. Omonse Talton who will guide you through developing a “fool proof” enzyme-linked immunosorbent assay (ELISA). In this webinar, you will learn: – When and why you should use the different types of ELISAs– direct, indirect, sandwich, competitive/inhibition ELISA – Major considerations for developing your ELISA – Tried and tested tips for you to perform a success...

In this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide mutagenesis screen to identify mutations responsible for resistance to the potent PARP inhibitor talazoparib (BMN 673). The screen yielded one particula...

We’ll discuss specific assays in each of these categories, the joys and pitfalls of each assay, and recommendations on how to choose the best method. You will learn tips and strategies for successful assay development using the following methods: Proliferation: 3H-Thymidine Uptake Bromodeoxyuridine Uptake (BrdU) ATP Luminescence Fluorescent Dye Reduction (CFSE) Cytokine Measurement: Multiplex vs....

While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In this webinar, you will learn: • Low (1-5 genes) vs medium (~300 genes) throughput experimental design • Pros and cons of self-designed vs “off the s...

Join us in this webinar featuring Dr. Vicki Doronina as she takes you through vital components of assay design. In this webinar, you will learn: How to choose between immortalized cells and primary cells for your assay How to avoid sources of bias in your cell-based assays How to use high throughput assays, so you can achieve greater reproducibility By now, you have heard about the reproducibility...

Join us for this webinar, featuring Dr. Marlon Stoeckius, as he explains how you can improve your single-cell RNA-sequencing (scRNA-seq) experiments. In this tutorial, you will find: How you can run one scRNA-seq experiment with numerous protein markers in parallel How you can increase your recovery of single cells (up to four times!) per experiment How you can link phenotypes to transcriptomic pr...

qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Generation Sequencing (NGS) has similar potential. However, the presence of large amounts of non-target DNA in most clinical or environmental samples precludes easy and inexpensive analysis of rare events. In this webinar, you will learn...

Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive observation of the interactions in the living cell is often impeded by principle limitations of conventional far-...

Elucidating meaningful, unbiased microbial community profiles from complex microbiome samples is challenging. In this webinar, you will learn: – the sources of bias throughout the microbiome analysis workflow – practical solutions for troubleshooting your techniques – new technologies to achieve the most representative and unbiased microbiome profiles Join Dr. Sven Reister as he guides you through...

Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the ratio of the donor and acceptor intensities (sensitized emission) or by detecting the excited state lifetime of the donor, which decreases with increasing FRET (Fluorescence Lifetime IMagi...

If image analysis is a place you fear to tread, or if you struggle with over complicated and time-consuming microscopy image analysis workflows, this is your opportunity to go beyond those limits. You will learn a fast, efficient and flexible approach to 4D microscopy image analysis, which yields high quality images and results. We’ll cover: • How to handle and process large image data quickly • H...

Current DNA synthesis and assembly technologies give today’s genetic engineers unprecedented freedom to control every aspect of genetic design.  In this webinar on DNA assembly, you will learn: Key concepts in DNA assembly The importance of analyzing combinatorial libraries of genetic designs for natural product biosynthesis Emerging areas of research Join Dr. Michael Smanski as he discusses his g...

In this webinar, you will learn about the advantages of deconvolving all your images fully automatically. Discussed in this webinar: – How to improve the quality of your images with the new Batch Express – How to increase resolution and signal of all your fluorescent microscopy data – How to deconvolve your new acquired images instantly – If you are interested in improving your fluorescent microsc...

In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn: The difference and benefits of LASSO cloning over NGS How LASSO cloning allows for the multiplex cloning of large open reading frames (ORFs) of bacterial, human, and human-microbiome genomes How this technology can impact our und...

Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as a very small pinhole to increase resolution while the overall detector design delivers better signal-to-noise than traditional GaAsP-based conf...

Join us in this webinar on demystifying DNA assembly. In this webinar, you will learn: – How flanking homology DNA assembly methods work – How to use web-based software to design experimental methods for flanking homology DNA assembly methods – How synthetic DNA fragments fit in to the DNA assembly process Dr. Nathan Hillson will discuss methods in flanking homology DNA assembly, including Gibson,...

In this webinar, you will learn about the power of 3D imaging of larger samples. In particular, you will: Gain insight in to how to use x-ray microscopy to analyze biological samples Learn the basics about sample preparation for x-ray microscopy Understand the benefits and drawbacks to different imaging conditions X-Ray Microscopy (XRM) is a relatively new technique that combines the geometric mag...

An unlikely renaissance is occurring in biological imaging, propelled by the ongoing development of serial blockface electron microscopy imaging technologies. Imaging systems based on scanning EM instruments place large-volume automated EM serial imaging within reach of most labs. How best to take advantage of the automation and versatility? Large scale connectomics studies are stunning in their s...

In this webinar, you will learn how to use optical imaging for functional cardiac physiological mapping of transmembrane potential, calcium handling, and metabolism. -The main aspects covered in the webinar include: -How to design an effective optocardiography system and select optimal fluorescence probes for your research needs using open source hardware and software. -Troubleshooting tips for re...

In this tutorial, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease We recently developed a technique that allows localized proteomics of microscopic regions of interest, such as specific cell types or neuropathological features of disease. This tech...

A major impediment in the determination of high resolution protein structures by single particle cryo-electron microscopy has been the presence of sample heterogeneity. Oftentimes, heterogeneity is due the dynamic nature of protein complexes, which can exist in multiple different conformational states in solution. Recent advancements in cryo-electron image processing have provided tools to accurat...

Recent advances in high-throughput multi-beam scanning electron microscopy (EM) and mouse whole-brain EM preparation and collection on tape (“Brain-on-Tape”) have resulted in substantial progress towards a nano-scale map of the whole mouse brain. These maps can be used to determine how individual neurons are synaptically connected and can be used to reconstruct the precise “wiring diagram” of the...