Let's Talk Micro

Those of you that work in Clinical Microbiology have seen at one point that a molecular test detects a gene for antimicrobial resistance and the susceptibility profile does not match. What do we do then? In this episode Dr. Rachael Liesman joins to the podcast to talk about this. What is genotypic testing? What is phenotypic testing? How do we perform these in the lab? How do we troubleshoot these...

This episode features an interview with the first graduate from the Doctorate in Clinical Laboratory Sciences (DCLS) program from Rutgers University: Dr. Brandy Gunsolus. What is the DCLS program? What are the requirements for this degree? What does a Doctor in Clinical laboratory Sciences does? Can they be directors? Tune in to find out about this career path for laboratorians and its contributio...

In this episode we talk about a relationship between bacteria and the Hawaiian bobtail squid. Dr. Sarah McAnulty and Dr. Spencer Nyholm join the podcast to talk about a study about this squid and bacteria. What is symbiosis? What is the accessory nidamental gland? How does a bacterial community contribute to the development of this gland? What bacteria make up this community? Tune in to find out....

The show "The last of us" has brought the topic of fungal infections to the spotlight. What is Cordyceps ? Why is the the term zombie used? What is the relationship between fungi and body temperature? Dr. Andrej Spec, an Infectious Diseases physician, joins the podcast to talk about Cordyceps and fungi.

This week's episode is about one species of Bacillus that causes disease and has the potential of being used as a bioterror agent : Bacillus anthracis . What is anthrax? How is this organism identified in the lab? What about Bacillus cereus biovar anthracis ? Tune in to this episode to find out the answers.

In this episode I continue going over Bacillus . Now that we know its groups and what diseases they are implicated on, it's time to talk about media and morphology. What do the colonies look like? What is MYP agar? PEMBA? Tune in to find out.

This week's episode is about Bacillus and related genera. What are the groups that are part of this genus? Which species cause food poisoning? What about endophthalmitis? What are spores? Tune in to find out. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

In a previous episode we talked about microbes in food. What about appliances such as a washing machine or a dishwasher? Or cleaning tools such as a sponge? Dr. Madeline Barron joins the podcast to answer those questions. She is the author of an article titled "Microbial Inhabitants of Cleaning Appliances and Tools". Tune in to find out what microbes are in those tools and appliances, recommendati...

This episode features an interview with Dr. Jose Alexander and Dustin Evans from AdvenHealth. They join the podcast to talk about a PCR test that they developed to detect amoeba. Not only it detects Naegleria fowleri , which is isolated in cases of Primary Amebic meningoencephalitis, but two other amoeba as well. Which ones does this test detect? Detecting amoeba in the laboratory is challenging d...

In this week's episode we go outside of Clinical Microbiology into Marine Microbiology. What better way to do that than talking with Dr. Jeanette Davis, a.k.a. Dr. Ocean. How do you become a Marine Microbiologist? What kind of work do they do? Besides her work, Dr. Davis is also a published author. Tune in to this episode to learn more! Dr. Ocean's website: https://drjeanettedavis.com

This episode features a conversation with Callum, an Infectious Disease physician and one of the co-hosts of ID:iots, an infectious disease podcast. What is their podcast about? What kind of work does an Infectious Disease doctor do? How do their work relates to the laboratory? Tune in for a great talk about the laboratory, challenges we have, and more. Link to ID:iots podcast: https://idiotspodca...

This episodes is about microbes in food. It features a conversation with Dr. Andrea Prinzi. Those of us that work in Microbiology are familiar with foods such as yogurt containing microbes. What about feta cheese? Enterococcus in your food? Tune in to find out more about this interesting topic that includes a popular meat substitute. Link to article: https://asm.org/Articles/2021/January/Microbes-...

One of the ways of assessing and reinforcing a Microbiology student's learning is by giving them unknown organisms. They typically get a broth containing the organism, and they plate it on agar, and perform a gram stain and biochemical testing to properly identify the organism. How can this concept be applied online? This is what this episode is about. Learn how two educators from the University o...

In this episode Dr. Andrea Prinzi returns to the podcast to talk about respiratory cultures. What are the types of respiratory cultures? What are the pathogens? What is the significance of yeast in respiratory cultures? Sputum rejection? Tune in to this episode to learn more about these cultures. Link to article about sputum rejection that Dr. Prinzi mentions in the episode: https://journals.asm.o...

This episode was originally released on February 24 of this year. It features an interview with Dr. Paul Luethy from the University of Maryland Medical Center. It is about a case of Wohlfarhtiimonas chitiniclastica that was seen in bacteremia. Typically this organism is seen in polymicrobial infections. It is being re-released for the benefit of new listeners so it complements the latest episode (...

This episode is about another case of Wohlfahrtiimonas chitiniclastica . It was presented in an article titled : "The fly who cried wolf" which was published by the American Society for Microbiology. Drs. David Gaston and Yembur Ahmad joined the podcast to discuss it. Tune in to listen to a great conversation about this organism, including morphology, biochemicals, and so much more. Link to articl...

Following the last episode about Yersinia species, on this episode I go over Y. pestis . Why is there concern about encountering Y. pestis in the laboratory? What do you do if you encounter potential bioterror agents? How does the laboratory handle those? As laboratories we are trained on how to recognize, test, handle, and ship these organisms. Tune in to this episode to learn about terms like La...

Following last week's episode about Y. pestis , what better than to learn about different species of Yersinia. What temperature range does Yersinisia grow at? Have you heard the term "bull's eye"? How about CIN agar? From enterocolitis to different forms of plague, tune in to this episode to lear about Yersinia , including media, biochemicals and more.

This episode features an interview with Philip Slavin and Maria Spyrou. They talk about Y. pestis and the second plague pandemic. A historical overview is provided and they talk about how DNA was obtained from 7 individuals buried in two cemeteries in the fourteenth century. Find out how this information points to an early source of the second plague pandemic. Link to article: https://www.nature.c...

This week Dr. Paul Hergenrother from the University of Illinois joins the podcast. Dr. Hergenrother talks about Fabimycin, an antibiotic candidate for gram negative bacteria. What is Fabimycin's mechanism of action? To which bacteria does it show activity against? Any intrinsic resistance? Tune in to find out. Link to article: https://pubs.acs.org/doi/10.1021/acscentsci.2c00598 Questions? Feedback...

This episode features an interview with Kathryn Lionetti and Heather Townsend. They are two Microbiology college professors. They implemented two ways of teaching microscopy remotely as a result of the pandemic. Just like other educators, they had to adapt. What do these methods consist of? How did the students perform? Tune in to find out. Link to article: https://doi.org/10.1128/jmbe.00332-21

Releasing on World Rabies Day (Sept. 28), this episode features an interview with Dr. Rodney Rohde, a Microbiologist and Professor and Chair of the Clinical Laboratory Science program at Texas State University. Dr. Rohde joins the podcast to talk about rabies. What is rabies? How do we acquire it? Prevention? Also, what is World Rabies Day? Tune in to find out. Dr. Rohde's rabies book and website...

This episode features an interview with Dr. Garcia- Ojeda from the University of California Merced. One of the results of the pandemic was that educators had to adapt to remote teaching. How do they remotely teach students those hands-on skills that are so important in Microbiology? Dr. Garcia Ojeda talks about what he did with his Microbiology laboratory. What was the outcome? How did the student...

In this episode I continue going over S. pneumoniae . This time I go over a popular antigen test, and I also discuss molecular testing, including limitations and the importance of correlating the gram stain with the result. Also, have you heard of Streptococcus pseudopneumoniae ?

This week I return to Streptococcus , this time S. pneumoniae . In this episode I go over its virulence, pathogenicity, and more. How do we identify it in the lab? What tests can we use? What is the bile solubility test? Tune in to this episode to find out.