Let's Talk Micro

Recorded live at ASM Microbe 2026 in Washington, DC, this special episode marks the first live recording of Let's Talk Micro. Joining me are co-host Dr. Andrea Prinzi and guest Dr. Nathan Ledeboer for a discussion on the evolving role of whole genome sequencing (WGS) in clinical microbiology and antimicrobial resistance. Can whole genome sequencing predict antimicrobial susceptibility? What are it...

En este episodio en español de Let's Talk Micro, Luis conversa nuevamente con el Dr. Germán Esparza sobre los cambios más relevantes incluidos en la edición 2026 del documento CLSI M100. Durante la conversación, revisan nuevas opciones terapéuticas como aztreonam-avibactam, actualizaciones en los puntos de corte para Acinetobacter y Neisseria gonorrhoeae , cambios en aminoglucósidos, consideracion...

ASM Microbe is the largest microbiology meeting in the United States, bringing together thousands of scientists, laboratorians, clinicians, and industry partners to discuss the latest advances in microbiology. In this episode, Luis shares highlights from ASM Microbe 2026, including sessions on diagnostic challenges, antimicrobial resistance, rapid susceptibility testing, emerging therapeutics, lab...

What does it take to go from working at the bench to becoming a future clinical microbiology laboratory director? In this episode, Luis is joined by Dr. Mackenzie Collins, a CPEP fellow at UCLA, to discuss her journey from Medical Laboratory Scientist (MLS) to PhD scientist and CPEP fellow. Mackenzie shares how she discovered laboratory medicine, her experience working as an MLS in molecular micro...

Have you ever received an identification result that just didn't make sense? In this episode, Luis shares three cases of mistaken identity from the microbiology laboratory: a Gram-positive rod identified as Kocuria, another reported as Sphingomonas, and a yeast that somehow became Brucella. While automated identification systems are powerful tools, they can only work with the information we provid...

In Part 2 of this hantavirus series, Luis continues the conversation with Dr. Ryan Relich and Dr. Stephanie Seifert by focusing on the recent Andes virus cruise ship outbreak and what makes this hantavirus different from others. The discussion explores the outbreak investigation, the evidence for person-to-person transmission, and why the Andes virus has received so much attention from the public...

Hantaviruses have recently been in the headlines, but what exactly are they and how do they spread? In Part 1 of this hantavirus series, Luis is joined by Dr. Ryan Relich and Dr. Stephanie Seifert for a deep dive into the world of hantaviruses. The conversation explores the basics of hantaviruses, including their ecology, rodent reservoirs, transmission, and the diseases they cause in humans. The...

Workforce shortages continue to impact clinical laboratories across the country, especially in rural healthcare settings. In this episode of Let's Talk Micro, Luis is joined by Kenzi Haigler to discuss an innovative high school laboratory program designed to introduce students to laboratory medicine early and help strengthen the future workforce pipeline. Kenzi shares her journey into the laborato...

In this episode of Let's Talk Micro, we close out our gram-negative cocci series by talking about the "background singers" of the group—the organisms that quietly show up on respiratory cultures and oral flora plates every day. We explore the world of non-pathogenic Neisseria species, along with rod-shaped and coccobacillary Neisseria and Moraxella that break the classic gram-negative diplococci r...

This week, Let's Talk Micro is pausing the Gram-negative cocci series to talk about one of the biggest microbiology meetings of the year: ASM Microbe 2026. Luis is joined by Dr. Bobbi Pritt and Dr. Robert Tibbetts to discuss what attendees can expect from this year's meeting in Washington, DC. The conversation covers the new meeting structure, important trends in clinical microbiology, sessions an...

In this episode of Let's Talk Micro, we continue our gram-negative cocci series with a closer look at Moraxella catarrhalis —better known at the bench as the hockey puck bug. We break down what makes this organism unique, from its classic sliding colony morphology to how it behaves in different patient populations. Is it a colonizer or a pathogen? The answer depends on the clinical context. We wal...

In this week's episode, we continue our series on gram-negative cocci and take a closer look at two of the most important organisms: Neisseria meningitidis and Neisseria gonorrhoeae. On the Gram stain, they may look the same—but clinically, they represent very different scenarios. We walk through colonization, transmission, and key virulence factors, and connect them to the infections they cause—f...

In this episode we continue our gram-negative cocci series by moving beyond the plate and into modern identification methods. From traditional systems like VITEK and RapID NH to MALDI-TOF and molecular testing, we explore how these organisms are identified in today's clinical microbiology lab. We also break down the role of NAATs and syndromic panels in detecting organisms like Neisseria meningiti...

In this episode of Let's Talk Micro, we continue our gram-negative cocci series by focusing on what to do after the Gram stain. You've identified gram-negative cocci—now what? We take a practical, bench-focused approach to working these organisms up, covering key culture media like blood agar, chocolate agar, and selective media such as Modified Thayer-Martin and Martin-Lewis. We also review colle...

In this episode of Let's Talk Micro, we kick off a new series on gram-negative cocci by starting where it all begins—the Gram stain. Seeing gram-negative diplococci on a smear can feel intimidating, especially when you're on the bench and need to make the right call. In this episode, we focus on how to approach that moment with confidence. We walk through the key organisms you'll encounter, includ...

The CLSI M100 gets updated every year—but what do those changes actually mean for the microbiology lab? In this episode of Let's Talk Micro, Luis is joined by Dr. April Bobenchik to break down the latest updates in the CLSI M100 2026 (36th edition) and how they translate into real-world lab practice. They walk through important breakpoint changes, including aztreonam–avibactam and updates for Acin...

Luis here with a quick update! We're taking a short break and will return the week of March 23rd. During this time, it's a great opportunity to explore the archive— Let's Talk Micro has over 200 episodes covering many topics in clinical microbiology. New episodes will be back soon. Thanks for listening and for being part of the Let's Talk Micro community. Stay connected with Let's Talk Micro: Webs...

Wound cultures are some of the most complex specimens encountered in the clinical microbiology laboratory. Unlike more straightforward cultures, wound specimens often contain multiple organisms, making it challenging to determine which microbes are clinically significant and which represent colonization. In this episode of Let's Talk Micro, Luis is joined by Dr. Christopher Doern and Dr. Alexandra...

The Gram stain is one of the most trusted tools in clinical microbiology—but it doesn't always tell the full story. In this Micro Minutes episode, we break down why Gram stains can sometimes be misleading and how factors like culture age, antibiotic exposure, decolorization, morphology, and arrangement can change what you see under the microscope. We also touch on classic troublemakers like Acinet...

In this episode of Let's Talk Micro, we step beyond the microbiology bench to talk laboratory medicine, advocacy, and storytelling with fellow medical laboratory scientists and podcasters Stephanie Whitehead and Aaron Odegard. We explore how their work on the SuperStat Podcast is helping amplify the voice of the laboratory through conversations on leadership, access, and visibility. The discussion...

In this episode of Let's Talk Micro, Luis is joined by Dr. Joel Mortensen to discuss a study examining intestinal parasites in pediatric patients in Guinea, West Africa. They dive into the real-world challenges of diagnosing parasitic infections in resource-limited settings, including reliance on stool microscopy, the use of rapid antigen tests, and how seasonal changes influence infection rates....

Genomic epidemiology is reshaping how we detect, investigate, and control infectious disease outbreaks—but what does that actually look like from the bench to public health? In this episode of Let's Talk Micro, Luis is joined by genomic epidemiologist Krisandra Allen for a practical, case-based conversation on how pathogen sequencing data is generated, analyzed, and paired with epidemiologic infor...

In this episode of Let's Talk Micro, Luis is joined by faculty and collaborators from the University of Florida to discuss their new Clinical Laboratory Microbiologist (CLM) program — the first NAACLS-approved, microbiology-only certification pathway in the United States. They share how the program was created to address the growing shortage of clinical microbiologists and provide a direct pathway...

Tick-borne diseases continue to expand across North America, but diagnosing them in the clinical laboratory remains complex—especially when timing between symptom onset and testing isn't considered. In this episode of Let's Talk Micro, Luis is joined by Kyle Rodino, Assistant Professor of Pathology and Laboratory Medicine at the University of Pennsylvania and Assistant Director of Microbiology, fo...

In this episode of Let's Talk Micro, we break down metagenomic next-generation sequencing (mNGS) and how it's changing the way we diagnose complex infectious diseases. I'm joined by Steve Miller, MD, PhD—Chief Medical Officer at Delve Bio—to discuss how unbiased metagenomics moved from research labs into real-world clinical practice. We cover what metagenomics is, how it differs from targeted PCR...