Luis Plaza
Let's Talk Micro
Clinical microbiology explained in simple terms, suitable for students and microbiologists alike. We discuss organisms, reactions, infectious diseases, and more. Hosted by Luis Plaza, a Medical Laboratory Scientist and microbiologist, who also shares his experiences in the field.
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Épisodes
73: Detecting amoeba: development of a PCR test 20.01.2023 27:21
This episode features an interview with Dr. Jose Alexander and Dustin Evans from AdvenHealth. They join the podcast to talk about a PCR test that they developed to detect amoeba. Not only it detects Naegleria fowleri , which is isolated in cases of Primary Amebic meningoencephalitis, but two other amoeba as well. Which ones does this test detect? Detecting amoeba in the laboratory is challenging d...
72: Marine Microbiology with Dr. Ocean 23.12.2022 28:10
In this week's episode we go outside of Clinical Microbiology into Marine Microbiology. What better way to do that than talking with Dr. Jeanette Davis, a.k.a. Dr. Ocean. How do you become a Marine Microbiologist? What kind of work do they do? Besides her work, Dr. Davis is also a published author. Tune in to this episode to learn more! Dr. Ocean's website: https://drjeanettedavis.com
71: Let's Talk Micro meets ID:iots 16.12.2022 1:01:26
This episode features a conversation with Callum, an Infectious Disease physician and one of the co-hosts of ID:iots, an infectious disease podcast. What is their podcast about? What kind of work does an Infectious Disease doctor do? How do their work relates to the laboratory? Tune in for a great talk about the laboratory, challenges we have, and more. Link to ID:iots podcast: https://idiotspodca...
70: Microbes and your grocery list 09.12.2022 31:47
This episodes is about microbes in food. It features a conversation with Dr. Andrea Prinzi. Those of us that work in Microbiology are familiar with foods such as yogurt containing microbes. What about feta cheese? Enterococcus in your food? Tune in to find out more about this interesting topic that includes a popular meat substitute. Link to article: https://asm.org/Articles/2021/January/Microbes-...
69: Online unknowns 02.12.2022 25:40
One of the ways of assessing and reinforcing a Microbiology student's learning is by giving them unknown organisms. They typically get a broth containing the organism, and they plate it on agar, and perform a gram stain and biochemical testing to properly identify the organism. How can this concept be applied online? This is what this episode is about. Learn how two educators from the University o...
68: Respiratory cultures 18.11.2022 41:40
In this episode Dr. Andrea Prinzi returns to the podcast to talk about respiratory cultures. What are the types of respiratory cultures? What are the pathogens? What is the significance of yeast in respiratory cultures? Sputum rejection? Tune in to this episode to learn more about these cultures. Link to article about sputum rejection that Dr. Prinzi mentions in the episode: https://journals.asm.o...
67: Re-release: Wohlfarhtiimonas chitiniclastica Monomicrobial bacteremia 16.11.2022 20:24
This episode was originally released on February 24 of this year. It features an interview with Dr. Paul Luethy from the University of Maryland Medical Center. It is about a case of Wohlfarhtiimonas chitiniclastica that was seen in bacteremia. Typically this organism is seen in polymicrobial infections. It is being re-released for the benefit of new listeners so it complements the latest episode (...
66: Wohlfahrtiimonas and the fly who cried Wohlf 11.11.2022 53:48
This episode is about another case of Wohlfahrtiimonas chitiniclastica . It was presented in an article titled : "The fly who cried wolf" which was published by the American Society for Microbiology. Drs. David Gaston and Yembur Ahmad joined the podcast to discuss it. Tune in to listen to a great conversation about this organism, including morphology, biochemicals, and so much more. Link to articl...
65: Yersinia pestis 03.11.2022 24:17
Following the last episode about Yersinia species, on this episode I go over Y. pestis . Why is there concern about encountering Y. pestis in the laboratory? What do you do if you encounter potential bioterror agents? How does the laboratory handle those? As laboratories we are trained on how to recognize, test, handle, and ship these organisms. Tune in to this episode to learn about terms like La...
64: Yersinia 27.10.2022 22:52
Following last week's episode about Y. pestis , what better than to learn about different species of Yersinia. What temperature range does Yersinisia grow at? Have you heard the term "bull's eye"? How about CIN agar? From enterocolitis to different forms of plague, tune in to this episode to lear about Yersinia , including media, biochemicals and more.
63: The source of the Black Death 20.10.2022 25:09
This episode features an interview with Philip Slavin and Maria Spyrou. They talk about Y. pestis and the second plague pandemic. A historical overview is provided and they talk about how DNA was obtained from 7 individuals buried in two cemeteries in the fourteenth century. Find out how this information points to an early source of the second plague pandemic. Link to article: https://www.nature.c...
62: Fabimycin: an antibiotic candidate 13.10.2022 21:28
This week Dr. Paul Hergenrother from the University of Illinois joins the podcast. Dr. Hergenrother talks about Fabimycin, an antibiotic candidate for gram negative bacteria. What is Fabimycin's mechanism of action? To which bacteria does it show activity against? Any intrinsic resistance? Tune in to find out. Link to article: https://pubs.acs.org/doi/10.1021/acscentsci.2c00598 Questions? Feedback...
61: Options for teaching microscopy remotely 06.10.2022 39:47
This episode features an interview with Kathryn Lionetti and Heather Townsend. They are two Microbiology college professors. They implemented two ways of teaching microscopy remotely as a result of the pandemic. Just like other educators, they had to adapt. What do these methods consist of? How did the students perform? Tune in to find out. Link to article: https://doi.org/10.1128/jmbe.00332-21
60: Learning about Rabies with Dr. Rodney Rohde 28.09.2022 53:19
Releasing on World Rabies Day (Sept. 28), this episode features an interview with Dr. Rodney Rohde, a Microbiologist and Professor and Chair of the Clinical Laboratory Science program at Texas State University. Dr. Rohde joins the podcast to talk about rabies. What is rabies? How do we acquire it? Prevention? Also, what is World Rabies Day? Tune in to find out. Dr. Rohde's rabies book and website...
59: Teaching Microbiology Remotely 22.09.2022 50:59
This episode features an interview with Dr. Garcia- Ojeda from the University of California Merced. One of the results of the pandemic was that educators had to adapt to remote teaching. How do they remotely teach students those hands-on skills that are so important in Microbiology? Dr. Garcia Ojeda talks about what he did with his Microbiology laboratory. What was the outcome? How did the student...
58: S. pneumoniae: testing and more 15.09.2022 22:43
In this episode I continue going over S. pneumoniae . This time I go over a popular antigen test, and I also discuss molecular testing, including limitations and the importance of correlating the gram stain with the result. Also, have you heard of Streptococcus pseudopneumoniae ?
57: Streptococcus pneumoniae 08.09.2022 22:08
This week I return to Streptococcus , this time S. pneumoniae . In this episode I go over its virulence, pathogenicity, and more. How do we identify it in the lab? What tests can we use? What is the bile solubility test? Tune in to this episode to find out.
56: Critical shortages in Microbiology 01.09.2022 37:29
This week's episode features an interview with Dr. Jeniffer Dien Bard and Dr. Amy Leber. Dr. Dien Bard is the Director of Microbiology at the Children's Hospital in Los Angeles, California. Dr. Leber works at the Department of Pathology Laboratory Medicine at Nationwide Children's Hospital in Columbus, Ohio. They join the podcast this week to talk about the shortages of Clinical Medical Laboratory...
55: Michigan Tech University-MLS Summer Youth Program 25.08.2022 32:08
This episode features an interview with Claire Danielson and Sarah LewAllen form the Medical Laboratory Sciences Program at Michigan Tech University (MTU). They discuss an initiative that has been in place for quite some time: the Summer Youth Program. This program brings awareness to the Medical Lab Science profession by teaching high school students about the laboratory.
Episode 54: VRE tests and media 01.07.2022 19:11
In this episode I go two types of media and one PCR test used for the detection of Vancomycin Resistant Enterococcus ( VRE ). What is this media composed of? How does it detect VRE? What about this PCR test? What is it looking for? Tune in to find out.
Episode 53: Enterococcus species 24.06.2022 23:20
In this episode I go over Enterococcus species. What are the most clinically significant species of Enterococcus ? Morphology? Biochemicals? Tune in to find out more about these organisms, and why are they so significant in healthcare settings.
Episode 52: Tips and suggestions in Microbiology part two 17.06.2022 23:38
In this episode I continue going over tips and suggestions you can use when you don't have supplies or are having difficulty isolating an organism. What if you are out of PEA or CNA? Will Lim broth help? What else can you use Martin Lewis and Modified Thayer Martin for ? How do susceptibilities help you isolate an organism? Tune in to find out.
Episode 51: Tips and suggestions in Microbiology 10.06.2022 24:18
In the laboratory we have all experienced a shortage of supplies. We have also struggled to isolate and organism. What do we do when this happens? What if you are trying to isolate a gram positive cocci but there is no PEA or CNA? What if you need to isolate a Pseudomonas aeruginosa from a mixed culture? Tune in to this episode to find out.
Episode 50: Lights, Camera… Lab! Dr. Alan Wu's Vision 02.06.2022 23:37
This episode features an interview with Dr. Alan Wu, Professor of Laboratory Medicine at the University of California, San Francisco, and co-Laboratory Director at Zuckerberg San Francisco General Hospital. Dr. Wu has made numerous contributions to the laboratory field—and now he's bringing the lab to television. He's working on a show designed to accurately depict the clinical laboratory and high...
Episode 49: Streptococcus anginosus group 27.05.2022 21:01
In this episode I continue going over beta hemolytic strep: this time Streptococcus anginosus group. What are the species that compose this group? What is the Lancefield group? What about biochemicals and morphology? Tune in to find out. Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: htt...
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