HistoTalks: NSH Podcasts

P08-Diagnostic Cytopathology Cell Block Preparation Methods- Anna Patterson FIBMS MSc CSci Diagnostic Cytology Scheme Coordinator Helen Naylor MSc Diagnostic Cytology Technical Specialist Cell blocks from Cytopathology samples have always had value in the diagnostic process as a complement to the traditional Cytopathology stains – Papanicolaou and Romanowsky.  This has become more important to pro...

P06-Understanding the Quality of your Electron Microscopy Provider in this Era of Outsourcing of Services- Tracey de Haro MSc, FIBMS, UK NEQAS CPT TEM Scheme Coordinator Specialist Scientific Lead for Electron Microscopy University Hospitals Of Leicester NHS Trust, UK  Background Electron Microscopy (EM) remains vital to the diagnostic repertoire for the diagnosis of pathologies. Surveys of diagno...

Research Requires Flexibility: Protease-Free Permeabilization Expands FISH Tissue Applications.-Andrelie Branicky, Shared Laboratory Resources, Lerner Research Institute, Cleveland Clinic, Cleveland, OH Fluorescence in situ hybridization (FISH) visualizes the presence of a specific DNA or RNA sequence in a tissue sample or cell. This method, particularly the mRNA version, detects gene expression w...

The Development of a Cocktail of Microglia and GFAP For Easy Diagnosis - Anisha Bhasin B.S, Sarah Holguin, MBA. B.S, Joe Vargas, M.S Microglia and GFAP are distinct neural markers, typically used separately to diagnose the degree of neurological infection and injury. Microglia, a glial cell, is used in the immune response of the central nervous system. GFAP is an astrocyte marker; astrocytes provi...

Study tools for the histotechnologist (HTL) Histotechnician (HT) certification exam- Amber Moser1, BS, Hannah Benton1, BS, Taylor Wallace1, BS, and Elizabeth A. Chlipala1, BS, HTL(ASCP)QIHC Premier Labs, Longmont, CO Histology laboratories have seen an increase in workplace shortages since the COVID-19 pandemic and an increase in noncertified applicants to fill open positions. The traditional rout...

Somagen Method of Alcohol Reagent Tracking (SMART) Optimization to Realize Workflow Efficiencies within the Laboratory-Anne de Wolf, BSc MLT Somagen Diagnostics, Edmonton, Alberta, Canada; Christine Chiu MLT Somagen Diagnostics, Toronto, Ontario, Canada; Annie Vallee Somagen Diagnostics, Montreal, Quebec, Canada; James Tang, BSc MLT Somagen Diagnostics, Edmonton, Alberta, Canada Tissue processing...

Method for FFPE Organ-Chip -Lindsay Parmelee HTL(ASCP) 1,2,3, Stephanie Pei Tung Yiu PhD 1,2,3,4, Chi Ngai Chan PhD 1,2,3, Sizun Jiang PhD 1,2,31 Center for Virology and Vaccine Research 2 Beth Israel Deaconess Medical Center 3 Harvard Medical School 4 Wyss Institute Organoids are currently being developed for applications in biomedicine such as drug development and disease research. With many org...

Assessing Adhesion Slide Performance Across Histology Applications -Colin Brewer, CellPath, Newtown, Wales; Rachel Finn, HTL, StatLab, McKinney, TX; Neil Haine, PhD, CellPath, Newtown, Wales; Arielle Hobson, StatLab, McKinney, TX; Moritz Kamphenkel, Knittel Glass, Braunschweig, Germany; Ronja-Melinda Komoll, Ph. D, Knittel Glass, Braunschweig, Germany; Racheal Moore, HT, StatLab, McKinney, TX; Nic...

The Use of Immunohistochemistry vs. qPCR to Detect BRAFV600E in Thyroid Cancer- Kannitha Chek HTL (ASCP), Nicholas Hoo-Fatt, MS, HTL (ASCP), DP, Samantha Diamond, M.D., Haresh Mani, M.D., Myong Ho “Lucy” Nam, M.D. Introduction: This study aimed to validate the effectiveness of the Ventana anti-BRAFV600E antibody in detecting the BRAFV600E mutation in thyroid cancer using immunohistochemistry (IHC)...

Whole NHP Brain Microtomy Technique- S Wessel, MS, HTL (ASCP),AskBio, Discovery 1, Morrisville, North Carolina T Marais, AskBio France, Paris Brain Institute (ICM), Paris, France M Martini, PhD, PhD, AskBio, Discovery 1, Morrisville, North Carolina, M Faridounnia, PhD, MSc, AskBio, Discovery 1, Morrisville, North Carolina, D Gregorowicz, HTL (ASCP),AskBio, Discovery 1, Morrisville, North Carolina,...

Encapsulated Cell Therapies in Sheep as a Translational Model - Trisha M. Fabijanic, B.S., University of Arizona, Tucson, AZ, Amy C. Kelly, Ph. D, University of Arizona, Tucson, AZ, Eliza H. Johnson, University of Arizona, Tucson, AZ, Delaney A. Drew, B.S., University of Arizona, Tucson, AZ, Demetri A. Vlachos, B.S., University of Arizona, Tucson, AZ, Charles W. Putnam, M.D., Ph. D., University of...

Cellular localization of the pink bollworm Cry1Ac Bt protein receptor PgCad1 in cultured insect cells Authors: Melanie Miranda (BS, HTL- ASCP, University of Arizona-Comparative Pathology Core Laboratory, Tucson, AZ) Jeff Fabrick (PhD, United States Department of Agriculture, Phoenix, AZ) Transgenic cotton genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringie...

2023 NSH co-presenters Gabriela De la Cruz and Lauren Ralph give great advice on selecting a topic and share why they decided to co-present at the 2023 NSH Convention. 

NSH member Ariel (Ari) Liberda discusses her decision to present at the 2023 NSH Convention.  She shares her journey and advice to anyone who is considering taking the leap!

Making the FNA Count Author: Jeffery Rinker The hospital at Sanford Bemidji Medical Center has 119 beds and performs an average of 150 FNAs each year. Our histologists do both histology and cytology, with FNAs comprising the majority of our cytology. Since 2020, our non-diagnostic rates on FNA samples have fluctuated. In 2022, our rate was as high as 28%. Compared to the non-diagnostic rate of 10%...

Alternative Strategies For Analyzing Pre-Clinical Mouse Lungs Authors: Nicholas Pankow, BA; Gabriela De la Cruz, BS; Hannah Marie Atkins, PhD, DVM In some diseases or conditions, it is challenging to leverage human tissues to determine patterns and resolve disease progression. Similarities between human and mouse biology make using murine models in pre-clinical studies possible. More specifically,...

BENEFITS of Tissue-Tek Paraform Sectionable Cassette System Authors: Jason (Jay) Innerhofer, PA(ASCP), M.H.S., Albuquerque, NM The Paraform System eliminates the need to perform manual steps at embedding and great in terms of training, histology and turn around time.

Leveraging preprocessor scanning to improve safety, quality, and lean workflow in the anatomic pathology lab. Authors: Lilly Guevara, Kristie Wolfe-Steele, Brian Johnson While procedures exist in AP laboratories to protect patient samples and prevent loss through specimen tracking, misplaced cassettes between grossing and tissue processing are still problematic. Significant time may be spent locat...

The Button Mushroom (Agaricus bisporus): An Alternative Control Source for the Grocott Methenamine Silver Technique Authors: From University of Texas M.D. Anderson Cancer Center Center, Houston, TX- Fatimah Ansar; Carla Arredondo; Tran Huynh; Victoria Jones; Jennifer Le; Duong Nguyen; Andres Ronquillo-Erazo; Minnu Varghese; Kaleena Ramirez; Toysha Mayer; Mark A. Bailey   Identifying the presence o...

 Gene Protein Assay(GPA): Unveiling Tumor Heterogeneity in HER2/neu Positive Breast Cancer Authors:  Dr. Tanuja Shet, Dr. Aditi Rathi    Introduction: HER2/neu gene amplification on Fluorescence in-situ hybridization (FISH) is defined as average HER2neu gene signals > 4 and HER2:CEP17 ratio of > 2, in breast cancer. However, with tumor heterogeneity interpretation of these cut-offs is challe...

Novel Use of Pipette Based Capsules for Glycol Methacrylate, Methyl/Butyl Methacrylate and Epoxy Resin Processing with Mouse Tissue That Reduce Processing Solution Volume and Time.   Authors: Philip Seifert, HTL(ASCP), Xinyao Hu, Bianai Fan and Darlene A. Dartt, PhD, Schepens Eye Research Institute of Mass Eye and Ear, Boston, MA   The mPrep/S capsules (Microscopy Innovations) are customized pipet...

Join us for our AwardsCast series as we have short conversations with the 2023 NSH Award & Scholarship Recipients. In this episode, we talk to Colleen Forster of the University of Minnesota, the recipient of the Immunohistochemistry Education Scholarship, where we discuss how every day in a core research lab is different and keeps her learning new things as well as her plans for learning more...

Join us for our AwardsCast series as we have short conversations with the 2023 NSH Award & Scholarship Recipients. In this episode, we talk to Cindy Lowe, HTL(ASCP), QIHC(ASCP), of Vanderbilt University, the recipient of the Jules Elias Award for Excellence in IHC & Molecular Techniques, where we discuss everything from her work in IHC, including an impactful visit from patients to her lab...

Join us for our AwardsCast series as we have short conversations with the 2023 NSH Award & Scholarship Recipients. In this episode, we talk to Beth Cox, CT (ASCP), HT(ASCP), HTL (ASCP), QIHC (ASCP), the recipient of the Helping Hands Scholarship for Histotechnology Outreach & Education where she discusses how she will use the funds to help identify and treat gynecologic cancer in women in...

Join us for our AwardsCast series as we have short conversations with the 2023 NSH Award & Scholarship Recipients. In this episode, we talk to Program Director, Taiquanda Winbush, from Albany State University about how they will use the funds to improve their program with the funds from the scholarship.