Dr Daniel J. Guerra

Authentic Biochemistry

Science EN ↓ 1250 episodes

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Dr Daniel J. Guerra

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Biochemical Mosaic I c.10. Is PE depletion reversed by mTORC1 phosphorylation of LIPIN1 or is DAG authentically activating CCT via membrane association thus obtaining robust PL synthesis? DJGPhD. 19.02.2024 30:00

References Biochim Biophys Acta. 2011 Jun;1811(6):377-85 Am J Physiol Lung Cell Mol Physiol 2001. 281: L98–L107. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1997. Volume 1348, Issues 1–2 , 4 Pages 79-90. Am J Physiol Lung Cell Mol Physiol 2001. Volume 281. Issue 1 July Pages L108-L118 JBC 1991. Volume 266, Issue 36 , 25 December. Pages 24503-24508 Progress in Lipid Research....

Biochemical Mosaic I. c.9 Increased inner mitochondrial membrane PE from PS decarboxylase decreases protease mediated degradation of phospholipid transfer proteins thus maintaining fusion potency. 19.02.2024 30:00

References PLoS Biol 2014. 12(10): e1001969 J Biol Chem. 2012 May18;287(21):17589-17597 J Biol Chem. 2012 Nov 23;287(48):40131-9 Nature 2019. volume 575, pages 361–365 Alleluia: Lobet den Herren.

Biochemical Mosaic I PA-phosphatase c.8. Membrane fusion and the dynamics of mitochondrial reduction vs. proliferation; the PA phosphatase in lipid metabolism. DJGPhD. 16.2.24 Authentic Biochemistry 17.02.2024 29:48

References Dr Guerra membrane lectures archive FASEB J. 2021 Jun; 35(6): e21620. Vivaldi, A. 1711. Concerto for 4 Violins in B minor RV 580 https://youtu.be/SY3Kxf7ZTeI?si=JLQXMtK06Y6Yfu_k

Biochemical Mosaic I. Phosphatidic acid Phosphatase. c.7. Mutational reversal of IDH1 leads to NADPH depletion concomitant with potent anti-PHD enantiomeric 2-hydroxyglutarate obtaining pseudohypoxia. 16.02.2024 30:00

References FEMS Microbiol Rev.1998. Oct;22(4):255-75 Discoveries(Craiova). 2017 Jul-Sep; 5(3): e77. J Cell Mol Med. 2015 Jul; 19(7): 1427–1440. Oncogene. 2017 Mar 23; 36(12): 1607–1618. Bach, JS. 1742. Kunst der Fuge , BWV 1080; Marta Czech https://youtu.be/p1Sq1HOYglU?si=2GMF7kf3dLW4rr2O Lennon and MCartney.1968. "Martha my Dear" https://youtu.be/RXawa90YU2s?si=dUPDtTdm4UqrgWit

Biochemical Mosaic I. The PA phosphatase c.6. Alteration of the TCA pathway intermediates and hypoxia/nutrient deficiency control HIF1α stability indirectly regulating IMM protein and lipid turnover. 13.02.2024 30:00

References Front. Oncol. 2017. 26 November. V.7. Oncogene. 2017 Mar 23; 36(12): 1607–1618. Schubert, F. 1819 Piano Quintet in A major, D. 667/ "Trout" https://youtu.be/syod5rkFdNY?si=5F3rexiou7dTifYg Hunter, R., Garcia , J. 1970 "Ripple" https://youtu.be/xofhZx9eWUQ?si=tpOAMzyW5qUy01P_

The Biochemical Mosaic I. Phosphatidic Acid Phosphatase c.5. Tumor cells adapt bioenergetic fuel economy toward cell immortalization via re-crafting of mitochondrial IM lipid speciation.DJGPhD.12Feb24 12.02.2024 30:00

References Cell Metabolism 2019. 30: 720–734 Nature 2019. 575: 361–365 Squire, C. 1978. "Onward" Yes.

Biochemical Mosaic I. Phosphatidic Acid Phosphatase c.4 GLN utilization and an IMM protease regulate mitochondrial involvement in cancer growth. DJGPhD. Authentic Biochemistry 10Feb24 11.02.2024 30:00

References Cell Death & Disease 2022. volume 13.Article number: 444. Nature 2019. volume 575, pages 361–365 Scientific Reports 2021. volume 11, Article number: 16512 Experimental & Molecular Medicine 2020. volume 52 , pages 1496–1516 Mozart, WA. 1788. Piano Trio in G Major, KV 564 https://youtu.be/oOEuVtr8sF0?si=3-9RK1XkGkSWne7I

Biochemical Mosaic Event Ontologies I. Phosphatidic Acid Phosphatase. c.3 Indirect consequences of mitochondrial proteomic variation potentially obtains GLN-linked Warburg glycolysis preventing ROS. 09.02.2024 29:48

References Cell Metabolism 2019. 30, 720–734 Redox Biology2013. Volume 1, Issue 1: 427-432 Nature 2019. volume 575. 361–365 Mozart, WA, 1779.Sinfonia Concertante Es-Dur, KV 364 https://youtu.be/iGDfj5uM_qA?si=bKNmj1r1o9zSMaA7 Lennon-McCartney. 1966. "Im Only Sleeping" https://youtu.be/sAG3m3p9UeI?si=oT6Luxnc6eFS_pEq

Biochemical Mosaic Event Ontologies I. Phosphatidic Acid Phosphatase. Autophagy commitment is linked to mTORC1 v. AMPK oppostional kinase networks maintaining proteostasis and lipid homeostasis.DJGPhD 09.02.2024 30:00

References Nature 2019.volume 575, pages 361–365 Cells 2022, 11(21), 3339 Guerra: Polypeptide, endomembranous lipid homeostasis, and bioenergetics lecture archives.

Biochemical Mosaic Event Ontologies I. Phosphatidic Acid Phosphatase. Prologomena c.1. 07.02.2024 30:00

References J Cell Biol. 2014 Mar 17; 204(6):919–929 Nature 2019. Volume 575, pages 361–365. Redox Biology 2013. Volume 1, Issue 1. Pages 427-432. Madrigali Guerrieri et Amorosi {Libro Ottavo} https://youtu.be/2TjkhWARBVE?si=-ZI_kVa5WZX_9qnB

BioMedical Portrait VIII. C.22 CODA. Microglial Leukotrienes in neurodegeneration.DJGPhD.04Feb2024. Authentic Biochemistry Podcast. 04.02.2024 30:00

References Acta Neuropathologica Communications 2020. volume 8, Article number: 129. J Lipid Res. 2019 Feb; 60(2): 219–226. Palestrina, G. 1562. Missa papae Marcelli https://youtu.be/KSmT4VIjEtI?si=6s7MtdTuH0fsURU5

BioMedical Portrait VIII. c.21 Lysopholpids like sphingolipids hydrogen bond to cholesterol mediating lipid membrane raft mobility. DJGPhD. 2Feb2024. Authentic Biochemistry Podcast 03.02.2024 29:13

References Biochimica et Biophysica Acta (BBA) - Biomembranes 2015. Volume 1848, Issue 3 , Pages 805-812 Soft Matter 21-Jan-2021 Am J Rhinol Allergy. 2015 Jan-Feb; 29(1): 35–40. Scientific Reports 2017. volume 7 , Article number: 39661. Antioxid Redox Signal. 2011 Aug 15;15(4): 1043–1083. Journal of Immunotoxicology. 2008. 5:4, 347-352 Mozart, W. A. 1773, October. Symphony No. 25 in G minor K.183....

BioMedical Portrait VIII C.20. Heterologous lipid sequence membrane domains rearrange according to LysoPL polarity after PLA2 mediated 20:4 removal and acetosalicylic acid alters fatty acid ordering. 01.02.2024 30:00

References Soft Matter , 2021, 17 , 2742-2752 BBA Biomembranes 2015 Volume 1848, Issue 3 , Pages 805-812 Mozart, WA. 1788. Divertimento in E Major. K 563. https://youtu.be/E8c83bpOVXo?si=AUrRoRH0C1ie2xd0

BioMed PortraitVIII.c.19. Membrane thermotropic phase transition enthalpic responses to Leukotriene synthesis in relation to cholesterol and lysophospholipid fluid dynamics.DJGPhD.31.1.24.Authentic BC 31.01.2024 29:48

References Dr Guerra: membrane lectures Balin, M.1966. "Coming Back to Me" Jefferson Airplane. Surrealistic Pillow.1967. https://youtu.be/y2f3tlgApHw?si=cTa1nhYw6kcycxUs Dylan (Zimmerman),R. 1962 "Girl from the North Country". w. Johnny Cash. https://youtu.be/Je4Eg77YSSA?si=eYXfJhwOQisGjPHW Schubert, F. 1828.Piano Sonata in B-flat major, D.960 https://youtu.be/lncNcNtGkJY?si=KR...

BioMed Portrait VIII.c.18Aspirin PGS (COX) inhibited membrane topological and integrated molecular dynamics: leukotriene –cytokine mediated asthmatic inflammation.DJGPhD.29.1.24. Authentic Biochem. 29.01.2024 29:51

References Biochimica et Biophysica Acta (BBA) - Biomembranes 2015. Volume 1848, Issue 3 . Pages 805-812. J Immunol. 2018 Feb 1; 200(3): 915–927. Am J Rhinol Allergy. 2015 Jan-Feb; 29(1): 35–40. Corelli,A. 1714. Concerto in D Major Op. 6 No. 4, https://youtu.be/3smZkpqXYHs?si=uwEdDkRv-bz-zJXW

Sententia non Fengo, concluding remarks. BioMedical PortraitVIII. (C.17). LTC4 and LTD4 interact with IL-33 inducing cytokine generation in lung ILC2s.DJGPhD.28.1.24 29.01.2024 29:57

References J Immunol. 2018 Feb 1; 200(3): 915–927 Fontana, GB. 1641 Violin Sonata 2. https://youtu.be/2kkpqJkYmms?si=w-JT4cbsn9hl7GSP

Sententia non Fengo: A minor prolegomenon to the purpose of Authentic Biochemistry. DJGPhD.28.1.24 28.01.2024 29:59

References Plotinus. 204-270. ""Neoplatonist philosophy:The Enneads Kant, I. 1790. Critique of Judgment. Beatles. 1969. Abbey Road. https://youtu.be/oolpPmuK2I8?si=BKTiNJmp-omJ8lJH Aleotti, V. 1575-1620. Audivi vocem de caelo https://youtu.be/CaTNXjzQANs?si=Zh3whogggigHmp4i

BioMed Portrait VIII. c.16. Coordinated complimentarity of cell cycle MEK kinase cascade with the IL33-ST2 and CysLT1R mediatedILC2 activation to promote type 2 immunopathology. DJGPhD.25.1.24 26.01.2024 30:00

References J Immunol. 2018 Feb 1; 200(3): 915–927 Cells. 2020 Jan; 9(1): 198. Cells. 2022 Apr; 11(7): 1105 Nature Comm. 2020. volume 11, Article number: 35 Schubert F. 1829. Fantasie in F minor. D940 https://youtu.be/UyjzqPPXDcw?si=XW_SMP7j-Kza8pmq Winwood, Capaldi and Wood. 1967 (Traffic). "Dear Mr Fantasy" https://youtu.be/dyMiUmrouZU?si=1ZSdXupm3WFBAxTz

BioMedical Portrait VIII.c.15. ILC associated hippocampal RNA-BP's exacerbate GLU neurotoxicity and regulate CysLT1R in lung ILC2's. DJGPhD. 23.1.24. 23.01.2024 29:58

References Nat Commun. 2022; 13: 4435. Nat Commun 2020; 11: 35 Hayward, J. 1971. "You Can Never Go Home" Moody Blues. https://youtu.be/mUhboF8d1x0?si=IX4iP_W0f3uR65qo Jagger, Richards, Oldham. 1964. "As Tears Go By". Marianne Faithful. https://youtu.be/S8EykQaZ8CU?si=X42TiNsveR7rwdHB

BioMedical Portrait VIII.c.14 The dialectical events between immune responses and leukotriene signaling are modulated by tissue-resident and circulating cell patterns via RNA binding proteins.DJGPhD. 22.01.2024 29:49

References Nat Commun. 2022; 13: 4435. Virchows Archiv: European Journal of Pathology, 2020. Vol. 476, Issue 2 Cell Death Differ. 2015 Apr; 22(5): 703–718 Pentangle.1971. Hunting Song. https://youtu.be/CwvpMGc_J-w?si=s6pS2agc5s1N0H2z Falconieri , Cima, Uccellini, Turini, Merula, et al. 1600-1650. Early Italian Baroque Ensemble. https://music.youtube.com/playlist?list=OLAK5uy_mQznM05seareQTK-s3j-1V...

BioMedical PortraitVIII.c.13 Leukotriene metabolism and reception is linked to innate like lymphocytes involved in airway inflammation and hyper-responsiveness-associated asthma.DJGPhD.19.1.24.AB. 20.01.2024 29:40

References J Immunol. 2009 Feb 1; 182(3): 1641–1647. Cell Reports 2015 10, 2043-2054DOI Virchows Archiv:European Journal of Pathology. 2020. 09456317, Vol. 476, Issue 2 Poe, EA. 1845. "the Raven" https://youtu.be/wAdQ3CcPHQU?si=2qpStYF2gdUJ4f35 Buddy Morrow and his Orchestra. 1960. "the Black Cat" https://youtu.be/9w_VL7oq-mE?si=XiorgTkvSGzJ4BK0

BioMedical Portrait VIII.c.12. 5-LOX associates with FLAP and PLA2 at the nuclear envelope to trigger and control ILC2 mediation of the allergic asthma AHR pathology. DJGPhD 17.1.24. Authentic Bioch. 18.01.2024 30:00

References Immunology. 2019 Apr; 156(4): 402–412. J Immunol. 2017 Aug 1; 199(3): 1096–1104 Front. Immunol., 06 June 2019 Mol Biol Cell. 2012 Nov 15; 23(22):4456–4464. Virchows Archiv: European Journal of Pathology, 09456317, Feb2020, Vol. 476, Issue 2 Pavana, Gagliarda, Ciaccona, Passacaglia 1535-1615. https://youtu.be/AY533zpCw74?si=224HuGs438aPHrrd

BioMedical Portrait VIII. C.11 Peptidyl leukotrieneR- mediated transcription of PTGS2and LOX15B with IL4/IL13 and IL33 in asthmatic lung airway constrictionin MDM's LM's and ILC2's DJGPhD.16.1.24 17.01.2024 29:35

References Immunol.2017 Aug 1; 199(3): 1096–1104. British J of Pharmacology. 2015. Volume172, Issue17: 4319-4330 Beethoven, L. 1826. String Quartet Op. 135 in F Major (#16) https://youtu.be/1hxpIQ3XhXA?si=65eTt7TlOZYCNsG8

BioMedical Portrait VIII. c.10. The LOX5 and LOX12/15 pathways for 20:4, 20:5 and 22:6produce over 50 unique molecular species of oxylipins and lipoxins with unique biological sequalae.DJGPhD.14.1.24. 15.01.2024 29:40

References Int. J. Mol. Sci. 2022, 23(23), 14883. Prostaglandins Other Lipid Mediators. 2023 Jun:166:106726. British J of Pharmacology. 2015. Volume172, Issue17. 4319-4330 Mozart, WA. 1791. Requiem. D Minor, K. 626 https://music.youtube.com/watch?v=RDegrbjfiWU&si=x-SSYDxEOv5Vc4xE Miller, S. 1977. Wintertime. https://youtu.be/HUv7pXgZ9NA?si=JzaZOWKLkPEMiyKj

BioMedical Portrait VIII. c.9. Initial detail of the LOX vs.COX isoform utilization of essential and other dietary PUFA's in complex leukotriene biosynthesis. DJGPhD.11Jan24.Authentic Biochemistry. 12.01.2024 30:00

References British J of Pharmacology 2015. Volume172, Issue17September Pages 4319-4330 Prostaglandins Other Lipid Mediators. 2023 Jun:166:106726. 1718. Concerto No. 4 in F minor , Op. 8, RV 297, "Winter" ( L'inverno )